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wizard sv gel and pcr clean-up system purification kit  (Promega)

 
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    Structured Review

    Promega wizard sv gel and pcr clean-up system purification kit
    Wizard Sv Gel And Pcr Clean Up System Purification Kit, supplied by Promega, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/wizard+sv+gel+and+pcr+clean-up+system+purification+kit/wizard+genomic+dna+purification+kit/pmc12280279-78-8-7
    Average 90 stars, based on 1 article reviews
    wizard sv gel and pcr clean-up system purification kit - by Bioz Stars, 2026-09
    90/100 stars

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    Related Articles

    Nested PCR:

    Article Title: Elongation capacity of polyunsaturated fatty acids in the annelid Platynereis dumerilii .
    Article Snippet: .. Both the first round and nested PCR consisted of an initial denaturing at 98°C for 3 min, followed by 40 cycles of denaturation at 98°C for 10 s, annealing at 58°C for 15 s, extension at 68°C for 2 min, and a final extension at 68°C for 10 min. PCR products containing the ORF sequences of the targeted genes (eloA, eloB and eloC) were purified on an agarose gel using the Wizard SV gel and PCR clean-up system purification kit (Promega). .. After restriction with the corresponding restriction enzymes (New England Biolabs, Ipswich, MA, USA) (electronic supplementary material, table S1) and purification as above, the PCR products were ligated (T4 DNA ligase, Promega) into similarly restricted pYES2.

    Article Title: Elongation capacity of polyunsaturated fatty acids in the annelid Platynereis dumerilii
    Article Snippet: .. Both the first round and nested PCR consisted of an initial denaturing at 98°C for 3 min, followed by 40 cycles of denaturation at 98°C for 10 s, annealing at 58°C for 15 s, extension at 68°C for 2 min, and a final extension at 68°C for 10 min. PCR products containing the ORF sequences of the targeted genes ( eloA , eloB and eloC ) were purified on an agarose gel using the Wizard SV gel and PCR clean-up system purification kit (Promega). .. After restriction with the corresponding restriction enzymes (New England Biolabs, Ipswich, MA, USA) (electronic supplementary material, table S1) and purification as above, the PCR products were ligated (T4 DNA ligase, Promega) into similarly restricted pYES2.

    Polymerase Chain Reaction:

    Article Title: Elongation capacity of polyunsaturated fatty acids in the annelid Platynereis dumerilii .
    Article Snippet: .. Both the first round and nested PCR consisted of an initial denaturing at 98°C for 3 min, followed by 40 cycles of denaturation at 98°C for 10 s, annealing at 58°C for 15 s, extension at 68°C for 2 min, and a final extension at 68°C for 10 min. PCR products containing the ORF sequences of the targeted genes (eloA, eloB and eloC) were purified on an agarose gel using the Wizard SV gel and PCR clean-up system purification kit (Promega). .. After restriction with the corresponding restriction enzymes (New England Biolabs, Ipswich, MA, USA) (electronic supplementary material, table S1) and purification as above, the PCR products were ligated (T4 DNA ligase, Promega) into similarly restricted pYES2.

    Article Title: Effects of physalis rugose mosaic and groundnut ringspot viruses, in single and double infections, on the development, yield and postharvest fruit characteristics of <i>Physalis peruviana</i> plants
    Article Snippet: The first author received a scholarship from the Conselho Nacional de Desenvolvimento Científico e Tecnológico (CNPq).. 1Departamento de Fitopatologia e Nematologia, Escola Superior de Agricultura Luiz de Queiroz, Universidade de São Paulo (USP), Piracicaba, Brazil 2Facultad de Ciencias Agrarias, Universidad Nacional de Asunción (FCA/ UNA), San Lorenzo, Paraguay 3Departamento de Agroindústria, Alimentos e Nutrição, Escola Superior de Agricultura Luiz de Queiroz, Universidade de São Paulo (USP), Piracicaba, Brazil

    Article Title: Functionally diverse front-end desaturases are widespread in the phylum Annelida.
    Article Snippet: .. The PCR product was purified using Wizard ® SV gel and PCR clean-up system purification kit (Promega) and further sequenced (DNA Sequencing Unit, IBMCP-CSIC-UPV, Valencia, Spain) to confirm the species identity. .. Full-length ORF sequences of the front-end desaturases from P. dumerilii (3 sequences), E. fetida (3) and S. lamarcki (1) were amplified using the high-fidelity Phusion® DNA polymerase (Thermo Fisher Scientific).

    Article Title: Pre‐infection of tomato plants carrying the <i>Sw‐5</i> gene with tomato chlorosis virus did not alter infection with groundnut ringspot virus in the field
    Article Snippet: Tomato (Solanum lycopersicum) is a vegetable with a high socioeconomic impact that is cultivated in Brazil and many other regions of the world.. In 2019, 54,537 hectares of tomato crops were cultivated in Brazil, producing approximately 3,917,967 tonnes, thus making the country the ninth largest producer (FAO, 2022).. In Brazil, tomatoes are grown mainly in the southeast and centralwest regions, with the states of Goiás, São Paulo and Minas Gerais accounting for 65% of the national production (IBGE, 2019).

    Article Title: Oral Vaccination against Lawsonia intracellularis Changes the Intestinal Microbiome in Weaned Piglets
    Article Snippet: .. The Wizard ® SV Gel and PCR Clean-Up System purification kit (Promega, Madison, WI, USA) were used to purify the PCR products. .. The Illumina MiSeq platform at Macrogen Inc. (Seoul, Korea) was used to sequence 16S rRNA gene amplicons.

    Article Title: Major Histocompatibility Complex Class I-Related Chain A (MICA) Allelic Variants Associate With Susceptibility and Prognosis of Gastric Cancer
    Article Snippet: .. The amplicons were purified using the Wizard SV Gel and PCR Clean-Up System Purification Kit (Promega, USA). .. The amplified products were sequenced on an ABI PRISM-3500 XL sequencer (Applied Biosystems, Foster City, CA).

    Article Title: Occurrence of cefotaxime-resistant and beta-lactamase-producing Enterobacterales in poultry from small-scale farms in the Copperbelt province of Zambia
    Article Snippet: .. Briefly, PCR products were purified using the Promega Wizard ® SV Gel and PCR Clean-Up System purification kit (Madison, WI, USA), according to the manufacturer’s instructions. .. Further, all cefotaxime-resistant isolates that were negative for yaiO were identified through 16S rRNA Sanger sequencing using BigDye Terminator v.3.1 (Applied Biosystems, Waltham, MA, USA).

    Article Title: Elongation capacity of polyunsaturated fatty acids in the annelid Platynereis dumerilii
    Article Snippet: .. Both the first round and nested PCR consisted of an initial denaturing at 98°C for 3 min, followed by 40 cycles of denaturation at 98°C for 10 s, annealing at 58°C for 15 s, extension at 68°C for 2 min, and a final extension at 68°C for 10 min. PCR products containing the ORF sequences of the targeted genes ( eloA , eloB and eloC ) were purified on an agarose gel using the Wizard SV gel and PCR clean-up system purification kit (Promega). .. After restriction with the corresponding restriction enzymes (New England Biolabs, Ipswich, MA, USA) (electronic supplementary material, table S1) and purification as above, the PCR products were ligated (T4 DNA ligase, Promega) into similarly restricted pYES2.

    Purification:

    Article Title: Elongation capacity of polyunsaturated fatty acids in the annelid Platynereis dumerilii .
    Article Snippet: .. Both the first round and nested PCR consisted of an initial denaturing at 98°C for 3 min, followed by 40 cycles of denaturation at 98°C for 10 s, annealing at 58°C for 15 s, extension at 68°C for 2 min, and a final extension at 68°C for 10 min. PCR products containing the ORF sequences of the targeted genes (eloA, eloB and eloC) were purified on an agarose gel using the Wizard SV gel and PCR clean-up system purification kit (Promega). .. After restriction with the corresponding restriction enzymes (New England Biolabs, Ipswich, MA, USA) (electronic supplementary material, table S1) and purification as above, the PCR products were ligated (T4 DNA ligase, Promega) into similarly restricted pYES2.

    Article Title: Effects of physalis rugose mosaic and groundnut ringspot viruses, in single and double infections, on the development, yield and postharvest fruit characteristics of <i>Physalis peruviana</i> plants
    Article Snippet: The first author received a scholarship from the Conselho Nacional de Desenvolvimento Científico e Tecnológico (CNPq).. 1Departamento de Fitopatologia e Nematologia, Escola Superior de Agricultura Luiz de Queiroz, Universidade de São Paulo (USP), Piracicaba, Brazil 2Facultad de Ciencias Agrarias, Universidad Nacional de Asunción (FCA/ UNA), San Lorenzo, Paraguay 3Departamento de Agroindústria, Alimentos e Nutrição, Escola Superior de Agricultura Luiz de Queiroz, Universidade de São Paulo (USP), Piracicaba, Brazil

    Article Title: Functionally diverse front-end desaturases are widespread in the phylum Annelida.
    Article Snippet: .. The PCR product was purified using Wizard ® SV gel and PCR clean-up system purification kit (Promega) and further sequenced (DNA Sequencing Unit, IBMCP-CSIC-UPV, Valencia, Spain) to confirm the species identity. .. Full-length ORF sequences of the front-end desaturases from P. dumerilii (3 sequences), E. fetida (3) and S. lamarcki (1) were amplified using the high-fidelity Phusion® DNA polymerase (Thermo Fisher Scientific).

    Article Title: Pre‐infection of tomato plants carrying the <i>Sw‐5</i> gene with tomato chlorosis virus did not alter infection with groundnut ringspot virus in the field
    Article Snippet: Tomato (Solanum lycopersicum) is a vegetable with a high socioeconomic impact that is cultivated in Brazil and many other regions of the world.. In 2019, 54,537 hectares of tomato crops were cultivated in Brazil, producing approximately 3,917,967 tonnes, thus making the country the ninth largest producer (FAO, 2022).. In Brazil, tomatoes are grown mainly in the southeast and centralwest regions, with the states of Goiás, São Paulo and Minas Gerais accounting for 65% of the national production (IBGE, 2019).

    Article Title: Oral Vaccination against Lawsonia intracellularis Changes the Intestinal Microbiome in Weaned Piglets
    Article Snippet: .. The Wizard ® SV Gel and PCR Clean-Up System purification kit (Promega, Madison, WI, USA) were used to purify the PCR products. .. The Illumina MiSeq platform at Macrogen Inc. (Seoul, Korea) was used to sequence 16S rRNA gene amplicons.

    Article Title: Major Histocompatibility Complex Class I-Related Chain A (MICA) Allelic Variants Associate With Susceptibility and Prognosis of Gastric Cancer
    Article Snippet: .. The amplicons were purified using the Wizard SV Gel and PCR Clean-Up System Purification Kit (Promega, USA). .. The amplified products were sequenced on an ABI PRISM-3500 XL sequencer (Applied Biosystems, Foster City, CA).

    Article Title: Occurrence of cefotaxime-resistant and beta-lactamase-producing Enterobacterales in poultry from small-scale farms in the Copperbelt province of Zambia
    Article Snippet: .. Briefly, PCR products were purified using the Promega Wizard ® SV Gel and PCR Clean-Up System purification kit (Madison, WI, USA), according to the manufacturer’s instructions. .. Further, all cefotaxime-resistant isolates that were negative for yaiO were identified through 16S rRNA Sanger sequencing using BigDye Terminator v.3.1 (Applied Biosystems, Waltham, MA, USA).

    Article Title: Elongation capacity of polyunsaturated fatty acids in the annelid Platynereis dumerilii
    Article Snippet: .. Both the first round and nested PCR consisted of an initial denaturing at 98°C for 3 min, followed by 40 cycles of denaturation at 98°C for 10 s, annealing at 58°C for 15 s, extension at 68°C for 2 min, and a final extension at 68°C for 10 min. PCR products containing the ORF sequences of the targeted genes ( eloA , eloB and eloC ) were purified on an agarose gel using the Wizard SV gel and PCR clean-up system purification kit (Promega). .. After restriction with the corresponding restriction enzymes (New England Biolabs, Ipswich, MA, USA) (electronic supplementary material, table S1) and purification as above, the PCR products were ligated (T4 DNA ligase, Promega) into similarly restricted pYES2.

    Agarose Gel Electrophoresis:

    Article Title: Elongation capacity of polyunsaturated fatty acids in the annelid Platynereis dumerilii .
    Article Snippet: .. Both the first round and nested PCR consisted of an initial denaturing at 98°C for 3 min, followed by 40 cycles of denaturation at 98°C for 10 s, annealing at 58°C for 15 s, extension at 68°C for 2 min, and a final extension at 68°C for 10 min. PCR products containing the ORF sequences of the targeted genes (eloA, eloB and eloC) were purified on an agarose gel using the Wizard SV gel and PCR clean-up system purification kit (Promega). .. After restriction with the corresponding restriction enzymes (New England Biolabs, Ipswich, MA, USA) (electronic supplementary material, table S1) and purification as above, the PCR products were ligated (T4 DNA ligase, Promega) into similarly restricted pYES2.

    Article Title: Elongation capacity of polyunsaturated fatty acids in the annelid Platynereis dumerilii
    Article Snippet: .. Both the first round and nested PCR consisted of an initial denaturing at 98°C for 3 min, followed by 40 cycles of denaturation at 98°C for 10 s, annealing at 58°C for 15 s, extension at 68°C for 2 min, and a final extension at 68°C for 10 min. PCR products containing the ORF sequences of the targeted genes ( eloA , eloB and eloC ) were purified on an agarose gel using the Wizard SV gel and PCR clean-up system purification kit (Promega). .. After restriction with the corresponding restriction enzymes (New England Biolabs, Ipswich, MA, USA) (electronic supplementary material, table S1) and purification as above, the PCR products were ligated (T4 DNA ligase, Promega) into similarly restricted pYES2.

    Virus:

    Article Title: Effects of physalis rugose mosaic and groundnut ringspot viruses, in single and double infections, on the development, yield and postharvest fruit characteristics of <i>Physalis peruviana</i> plants
    Article Snippet: The first author received a scholarship from the Conselho Nacional de Desenvolvimento Científico e Tecnológico (CNPq).. 1Departamento de Fitopatologia e Nematologia, Escola Superior de Agricultura Luiz de Queiroz, Universidade de São Paulo (USP), Piracicaba, Brazil 2Facultad de Ciencias Agrarias, Universidad Nacional de Asunción (FCA/ UNA), San Lorenzo, Paraguay 3Departamento de Agroindústria, Alimentos e Nutrição, Escola Superior de Agricultura Luiz de Queiroz, Universidade de São Paulo (USP), Piracicaba, Brazil

    Article Title: Pre‐infection of tomato plants carrying the <i>Sw‐5</i> gene with tomato chlorosis virus did not alter infection with groundnut ringspot virus in the field
    Article Snippet: Tomato (Solanum lycopersicum) is a vegetable with a high socioeconomic impact that is cultivated in Brazil and many other regions of the world.. In 2019, 54,537 hectares of tomato crops were cultivated in Brazil, producing approximately 3,917,967 tonnes, thus making the country the ninth largest producer (FAO, 2022).. In Brazil, tomatoes are grown mainly in the southeast and centralwest regions, with the states of Goiás, São Paulo and Minas Gerais accounting for 65% of the national production (IBGE, 2019).

    DNA Sequencing:

    Article Title: Functionally diverse front-end desaturases are widespread in the phylum Annelida.
    Article Snippet: .. The PCR product was purified using Wizard ® SV gel and PCR clean-up system purification kit (Promega) and further sequenced (DNA Sequencing Unit, IBMCP-CSIC-UPV, Valencia, Spain) to confirm the species identity. .. Full-length ORF sequences of the front-end desaturases from P. dumerilii (3 sequences), E. fetida (3) and S. lamarcki (1) were amplified using the high-fidelity Phusion® DNA polymerase (Thermo Fisher Scientific).



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